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Homepage>BS Standards>65 AGRICULTURE>65.120 Animal feeding stuffs>BS EN 16936:2017 Animal feeding stuffs. Methods of sampling and analysis. Screening on the antibiotics tylosin, virginiamycin, spiramycin, bacitracin-zinc and avoparcin at sub-additive levels in compound feed by a microbiological plate test
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immediate downloadReleased: 2017-06-21
BS EN 16936:2017 Animal feeding stuffs. Methods of sampling and analysis. Screening on the antibiotics tylosin, virginiamycin, spiramycin, bacitracin-zinc and avoparcin at sub-additive levels in compound feed by a microbiological plate test

BS EN 16936:2017

Animal feeding stuffs. Methods of sampling and analysis. Screening on the antibiotics tylosin, virginiamycin, spiramycin, bacitracin-zinc and avoparcin at sub-additive levels in compound feed by a microbiological plate test

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Standard number:BS EN 16936:2017
Pages:20
Released:2017-06-21
ISBN:978 0 580 91796 7
Status:Standard
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BS EN 16936:2017


This standard BS EN 16936:2017 Animal feeding stuffs. Methods of sampling and analysis. Screening on the antibiotics tylosin, virginiamycin, spiramycin, bacitracin-zinc and avoparcin at sub-additive levels in compound feed by a microbiological plate test is classified in these ICS categories:
  • 65.120 Animal feeding stuffs
This European Standard presents a method describing the screening on the antibiotics tylosin, virginiamycin, spiramycin, bacitracin-zinc and avoparcin at sub-additive levels in complete feeding stuffs and milk replacers by a microbiological 3-plate test. The limit of detection of the method is 1 mg/kg for avoparcin, tylosin, spiramycin and virginiamycin, and 5 mg/kg for zinc bacitracin. The presence of other (veterinary) antibiotics may interfere with the method. Furthermore, high concentrations of metals (Cu, Zn) may interfere. The method should be used as a qualitative screening method. Positive results can be analysed further by TLC; for confirmatory purposes LC-MS is required [1]. A lower limit of detection for zinc bacitracin (3 mg/kg) is achievable (see Table 2), but should be established with an in house validation first.