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Homepage>ISO Standards>ISO 21115 Water quality — Determination of acute toxicity of water samples and chemicals to a fish gill cell line (RTgill-W1)
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download between 0-24 hoursReleased: 2019
ISO 21115 Water quality — Determination of acute toxicity of water samples and chemicals to a fish gill cell line (RTgill-W1)

ISO 21115

Water quality — Determination of acute toxicity of water samples and chemicals to a fish gill cell line (RTgill-W1)

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LANGUAGE
English
French
Standard´s number:ISO 21115
Pages:38
Edition:1
Released:2019
DESCRIPTION

ISO 21115


This document specifies a method for the determination of fish acute toxicity using the permanent cell line from rainbow trout (Oncorhynchus mykiss) gill, RTgill-W1. Cells in confluent monolayers in 24-well tissue culture plates are exposed to water samples, such as surface waters or different kinds of effluents, or to chemicals for 24 h and, thereafter, cell viability is assessed based on fluorescent cell viability indicator dyes (see 4.1). Data are then expressed as a percentage of unexposed control and toxicity quantified based on the percentage of cell viability versus the percentage of effluent or the chemical concentration in response curves (see Clause 9).
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