PRICES include / exclude VAT
Homepage>ISO Standards>ISO 21115:2019-Water quality-Determination of acute toxicity of water samples and chemicals to a fish gill cell line (RTgill-W1)
Sponsored link
download between 0-24 hoursReleased: 2019
ISO 21115:2019-Water quality-Determination of acute toxicity of water samples and chemicals to a fish gill cell line (RTgill-W1)

ISO 21115:2019

ISO 21115:2019-Water quality-Determination of acute toxicity of water samples and chemicals to a fish gill cell line (RTgill-W1)

Format
Availability
Price and currency
English PDF
Immediate download
233.33 USD
English Hardcopy
In stock
233.33 USD
French PDF
Immediate download
233.33 USD
French Hardcopy
In stock
233.33 USD
Standard´s number:ISO 21115:2019
Pages:38
Edition:1
Released:2019
DESCRIPTION

ISO 21115:2019


This document specifies a method for the determination of fish acute toxicity using the permanent cell line from rainbow trout (Oncorhynchus mykiss) gill, RTgill-W1. Cells in confluent monolayers in 24-well tissue culture plates are exposed to water samples, such as surface waters or different kinds of effluents, or to chemicals for 24 h and, thereafter, cell viability is assessed based on fluorescent cell viability indicator dyes (see 4.1). Data are then expressed as a percentage of unexposed control and toxicity quantified based on the percentage of cell viability versus the percentage of effluent or the chemical concentration in response curves (see Clause 9).