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Homepage>ISO Standards>ISO 21446:2019-Infant formula and adult nutritionals-Determination of trans and total (cis + trans) vitamin K1 content
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download between 0-24 hoursReleased: 2019
ISO 21446:2019-Infant formula and adult nutritionals-Determination of trans and total (cis + trans) vitamin K1 content

ISO 21446:2019

ISO 21446:2019-Infant formula and adult nutritionals-Determination of trans and total (cis + trans) vitamin K1 content

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Standard´s number:ISO 21446:2019
Pages:20
Edition:1
Released:2019
Language:English
DESCRIPTION

ISO 21446:2019


This document specifies a method for the quantitative determination of trans and total (cis + trans) vitamin K1 in infant, pediatric and adult nutritionals using normal phase (NP) high-performance liquid chromatography (HPLC) with post-column reduction and fluorescence detection. The method demonstrated good linearity over a standard range of ~2 μg/l to 80 μg/l trans vitamin K1, and the limit of quantification (LOQ) was estimated to be 0,4 μg/l for standards and 0,09 μg/100 g ready to feed (RTF) for samples assuming 4 grams of sample are diluted to 10 ml.