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Homepage>BS Standards>13 ENVIRONMENT. HEALTH PROTECTION. SAFETY>13.060 Water quality>13.060.70 Examination of biological properties of water>BS ISO 21115:2019 Water quality. Determination of acute toxicity of water samples and chemicals to a fish gill cell line (RTgill-W1)
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immediate downloadReleased: 2019-04-09
BS ISO 21115:2019 Water quality. Determination of acute toxicity of water samples and chemicals to a fish gill cell line (RTgill-W1)

BS ISO 21115:2019

Water quality. Determination of acute toxicity of water samples and chemicals to a fish gill cell line (RTgill-W1)

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Standard number:BS ISO 21115:2019
Pages:48
Released:2019-04-09
ISBN:978 0 580 98655 0
Status:Standard
DESCRIPTION

BS ISO 21115:2019


This standard BS ISO 21115:2019 Water quality. Determination of acute toxicity of water samples and chemicals to a fish gill cell line (RTgill-W1) is classified in these ICS categories:
  • 13.060.70 Examination of biological properties of water

This document specifies a method for the determination of fish acute toxicity using the permanent cell line from rainbow trout (Oncorhynchus mykiss) gill, RTgill-W1. Cells in confluent monolayers in 24-well tissue culture plates are exposed to water samples, such as surface waters or different kinds of effluents, or to chemicals for 24 h and, thereafter, cell viability is assessed based on fluorescent cell viability indicator dyes (see 4.1). Data are then expressed as a percentage of unexposed control and toxicity quantified based on the percentage of cell viability versus the percentage of effluent or the chemical concentration in response curves (see Clause 9).