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Homepage>BS Standards>67 FOOD TECHNOLOGY>67.050 General methods of tests and analysis for food products>BS ISO 21446:2019 Infant formula and adult nutritionals. Determination of <i>trans</i> and total (<i>cis</i> + <i>trans</i>) vitamin K<sub>1</sub> content. Normal phase HPLC
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immediate downloadReleased: 2019-01-09
BS ISO 21446:2019 Infant formula and adult nutritionals. Determination of <i>trans</i> and total (<i>cis</i> + <i>trans</i>) vitamin K<sub>1</sub> content. Normal phase HPLC

BS ISO 21446:2019

Infant formula and adult nutritionals. Determination of <i>trans</i> and total (<i>cis</i> + <i>trans</i>) vitamin K<sub>1</sub> content. Normal phase HPLC

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Standard number:BS ISO 21446:2019
Pages:28
Released:2019-01-09
ISBN:978 0 580 96185 4
Status:Standard
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BS ISO 21446:2019


This standard BS ISO 21446:2019 Infant formula and adult nutritionals. Determination of trans and total (cis + trans) vitamin K1 content. Normal phase HPLC is classified in these ICS categories:
  • 67.050 General methods of tests and analysis for food products

This document specifies a method for the quantitative determination of trans and total (cis + trans) vitamin K1 in infant, pediatric and adult nutritionals using normal phase (NP) high-performance liquid chromatography (HPLC) with post-column reduction and fluorescence detection. The method demonstrated good linearity over a standard range of ~2 μg/l to 80 μg/l trans vitamin K1, and the limit of quantification (LOQ) was estimated to be 0,4 μg/l for standards and 0,09 μg/100 g ready to feed (RTF) for samples assuming 4 grams of sample are diluted to 10 ml.