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Homepage>ISO Standards>ISO 11063 Soil quality — Direct extraction of soil DNA
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download between 0-24 hoursReleased: 2020
ISO 11063 Soil quality — Direct extraction of soil DNA

ISO 11063

Soil quality — Direct extraction of soil DNA

CURRENCY
LANGUAGE
English
French
Standard´s number:ISO 11063
Pages:10
Edition:2
Released:2020
DESCRIPTION

ISO 11063


The present document specifies a method for direct extraction of DNA from soil samples to analyse the abundance and composition of microbial communities by various techniques of molecular biology including real-time quantitative PCR (qPCR). This method is mainly dedicated to agricultural and forest soils. This method can possibly not be suitable for soils rich in organic matter (e.g. peat soils) or soils heavily polluted with organic pollutants or heavy metals. The direct extraction of DNA from soil samples provides unique insight into the α- and β-diversity of microbial communities. Next-generation sequencing of amplicons obtained by PCR (polymerase chain reaction) amplification of soil DNA constitutes a promising domain which will in the near future contribute to the development of routine tools to monitor microbial communities in soil environments.
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