ASTM E3481-25

1.1 This practice is used to evaluate the bacterial and fungal inhibition properties of a continuous-use vapor releasing test substance under simulated field conditions for use on hard, non-porous surfaces in controlled temperature and relative humidity levels.

1.2 This practice involves the use of hazardous materials, chemicals, and infectious microorganisms and therefore should be performed only by those trained in microbiological techniques in facilities designed and equipped for work with infectious agents at the appropriate biosafety level; a BSL-2 or higher laboratory. Specifications are provided in “Biosafety for Biomedical and Microbiological Laboratories” (BMBL).

1.3 It is the responsibility of the investigator to determine whether good laboratory practices (GLP) standards are met. For example, 40 CFR, Part 160 of FIFRA are required and to be followed when appropriate.

1.4 Strict adherence to the protocol is necessary for the validity of the test results.

1.5 Units—The values stated in SI units are to be regarded as the standard. No other units of measurement are included in this standard.

1.6 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety, health, and environmental practices and determine the applicability of regulatory limitations prior to use.

1.7 This international standard was developed in accordance with internationally recognized principles on standardization established in the Decision on Principles for the Development of International Standards, Guides and Recommendations issued by the World Trade Organization Technical Barriers to Trade (TBT) Committee.