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>ASTM Standards>ASTM E3512-25 - Standard Test Method for Quantitatively Evaluating the Efficacy of Antimicrobial Products on Hard, Non-Porous Surfaces Against Bacteria
Released: 01.09.2025

ASTM E3512-25

Standard Test Method for Quantitatively Evaluating the Efficacy of Antimicrobial Products on Hard, Non-Porous Surfaces Against Bacteria

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Standard number:ASTM E3512-25
Released:01.09.2025
Status:Active
Pages:14
Section:11.08
Keywords:antimicrobial test substance; bacteria; hard surface; Pseudomonas aeruginosa; quantitative efficacy test; Salmonella enterica; Staphylococcus aureus;
DESCRIPTION

1.1 This test method provides a quantitative assessment of the performance of liquid antimicrobial test substances including concentrated liquids, ready-to-use liquids, gels, sprays (aerosol, pressurized spray, trigger, pump, and foaming spray dispensers), and water-soluble powders (including water-soluble tablets) against Pseudomonas aeruginosa, Staphylococcus aureus, and Salmonella enterica designed for use on hard, non-porous surfaces. The test method may be appropriately modified to accommodate organisms not defined herein; however, the precision and bias statements do not apply.

1.2 This test method uses log reduction (LR) as the quantitative measure of efficacy for antimicrobial test substances against test microbes on a hard, non-porous surface.

1.3 This test method is based on principles established for Test Method E2197 and an OECD Guidance Document.

1.4 Training in basic microbiology and aseptic technique is required to perform this assay.

1.5 The values stated in SI units are to be regarded as standard. No other units of measurement are included in this standard.

1.6 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety, health, and environmental practices and determine the applicability of regulatory limitations prior to use.

1.7 This international standard was developed in accordance with internationally recognized principles on standardization established in the Decision on Principles for the Development of International Standards, Guides and Recommendations issued by the World Trade Organization Technical Barriers to Trade (TBT) Committee.