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Homepage>BS Standards>07 MATHEMATICS. NATURAL SCIENCES>07.100 Microbiology>07.100.30 Food microbiology>BS EN ISO 6579-4:2025 Microbiology of the food chain. Horizontal method for the detection, enumeration and serotyping of Salmonella Identification of monophasic Salmonella Typhimurium (1,4,[5],12:i:-) by polymerase chain reaction (PCR)
immediate downloadReleased: 2025-02-07
BS EN ISO 6579-4:2025 Microbiology of the food chain. Horizontal method for the detection, enumeration and serotyping of Salmonella Identification of monophasic Salmonella Typhimurium (1,4,[5],12:i:-) by polymerase chain reaction (PCR)

BS EN ISO 6579-4:2025

Microbiology of the food chain. Horizontal method for the detection, enumeration and serotyping of Salmonella Identification of monophasic Salmonella Typhimurium (1,4,[5],12:i:-) by polymerase chain reaction (PCR)

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Standard number:BS EN ISO 6579-4:2025
Pages:42
Released:2025-02-07
ISBN:978 0 539 29261 9
Status:Standard
BS EN ISO 6579-4:2025 - Microbiology of the Food Chain

BS EN ISO 6579-4:2025: Microbiology of the Food Chain

Horizontal Method for the Detection, Enumeration, and Serotyping of Salmonella

Introducing the BS EN ISO 6579-4:2025, a comprehensive standard that sets the benchmark for the detection, enumeration, and serotyping of Salmonella in the food chain. This essential document is a must-have for laboratories, food safety professionals, and microbiologists who are committed to ensuring the highest standards of food safety and public health.

Key Features and Benefits

This standard provides a detailed methodology for the identification of monophasic Salmonella Typhimurium (1,4,[5],12:i:-) using the advanced technique of polymerase chain reaction (PCR). Here are some of the key features and benefits:

  • Comprehensive Coverage: Spanning 42 pages, this standard offers an in-depth exploration of the horizontal method for Salmonella detection, ensuring you have all the information you need at your fingertips.
  • Latest Release: Released on February 7, 2025, this document reflects the most current research and technological advancements in the field of microbiology.
  • International Standard: As a part of the ISO series, this standard is recognized globally, facilitating international trade and cooperation in food safety.
  • Precision and Accuracy: The use of PCR technology ensures high precision and accuracy in the identification of Salmonella, reducing the risk of false positives and negatives.

Why Choose BS EN ISO 6579-4:2025?

In the ever-evolving landscape of food safety, staying ahead of potential risks is crucial. The BS EN ISO 6579-4:2025 standard equips you with the tools and knowledge to effectively monitor and control Salmonella in the food chain. By implementing this standard, you can:

  • Enhance Food Safety: Protect consumers by ensuring that food products are free from harmful levels of Salmonella.
  • Meet Regulatory Requirements: Comply with national and international food safety regulations, avoiding costly penalties and recalls.
  • Boost Consumer Confidence: Demonstrate your commitment to quality and safety, enhancing your brand's reputation and consumer trust.

Technical Specifications

For those who require detailed technical information, here are the specifications of the BS EN ISO 6579-4:2025 standard:

  • Standard Number: BS EN ISO 6579-4:2025
  • Pages: 42
  • Release Date: February 7, 2025
  • ISBN: 978 0 539 29261 9
  • Status: Standard

Who Should Use This Standard?

This standard is designed for a wide range of professionals involved in food safety and microbiology, including:

  • Food Safety Laboratories: Ensure accurate detection and analysis of Salmonella in food samples.
  • Quality Assurance Teams: Implement robust testing protocols to maintain product safety and quality.
  • Regulatory Bodies: Develop and enforce food safety regulations based on the latest scientific standards.
  • Academic Researchers: Conduct cutting-edge research in food microbiology and safety.

Conclusion

The BS EN ISO 6579-4:2025 standard is an indispensable resource for anyone involved in the food industry. By adopting this standard, you are taking a proactive step towards safeguarding public health and ensuring the integrity of the food supply chain. Stay ahead of the curve with the latest methodologies and ensure your operations meet the highest standards of safety and quality.

DESCRIPTION

BS EN ISO 6579-4:2025


This standard BS EN ISO 6579-4:2025 Microbiology of the food chain. Horizontal method for the detection, enumeration and serotyping of Salmonella is classified in these ICS categories:
  • 07.100.30 Food microbiology
This document specifies a horizontal in vitro method for the molecular identification and differentiation of the monophasic variant of Salmonella enterica subsp. enterica serovar Typhimurium (1,4,[5],12:i:-) lacking the second H phase H:1,2, starting from isolates. The method detects specific DNA sequences of an intergenic region of the first H phase flagellin cluster for identification of Salmonella enterica subsp. enterica serovar Typhimurium (further called Salmonella Typhimurium) and specific DNA sequences of genes associated with second H phase flagellar antigen expression. The method is applicable for: —     differentiation of the isolate under analysis between monophasic Salmonella Typhimurium and the monophasic variant of another Salmonella non-Typhimurium serovar that has the same antigenic formula; —     identification of the isolate under analysis being either monophasic Salmonella Typhimurium or (biphasic) Salmonella Typhimurium. This document is applicable for the analysis of a pure culture belonging to the genus Salmonella, isolated from: —     products intended for human consumption; —     products intended for animal feeding; —     environmental samples in the area of food and feed production and handling; —     samples from the primary production stage. This document can also be applied in other domains for identification of monophasic Salmonella Typhimurium (e.g. environmental, human health, animal health). NOTE            This method has been validated in a method evaluation study and in an interlaboratory study with a large set of different strains (target and non-target strains), isolated from different sources (food products, animals, animal feed, primary production samples and humans). For detailed information on the validation, see Annex E.