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Homepage>BS Standards>13 ENVIRONMENT. HEALTH PROTECTION. SAFETY>13.060 Water quality>13.060.70 Examination of biological properties of water>BS ISO 19040-1:2018 Water quality. Determination of the estrogenic potential of water and waste water Yeast estrogen screen (<i>Saccharomyces</i> <i>cerevisiae</i>)
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BS ISO 19040-1:2018 Water quality. Determination of the estrogenic potential of water and waste water Yeast estrogen screen (<i>Saccharomyces</i> <i>cerevisiae</i>)

BS ISO 19040-1:2018

Water quality. Determination of the estrogenic potential of water and waste water Yeast estrogen screen (<i>Saccharomyces</i> <i>cerevisiae</i>)

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409.41 USD
Standard number:BS ISO 19040-1:2018
Pages:60
Released:2018-08-10
ISBN:978 0 580 84720 2
Status:Standard
DESCRIPTION

BS ISO 19040-1:2018


This standard BS ISO 19040-1:2018 Water quality. Determination of the estrogenic potential of water and waste water is classified in these ICS categories:
  • 13.060.70 Examination of biological properties of water

This document specifies a method for the determination of the estrogenic potential of water and waste water by means of a reporter gene assay with genetically modified yeast strains Saccharomyces cerevisiae. This reporter gene assay is based on the activation of the human estrogen receptor alpha.

This method is applicable to:

  • fresh water;

  • waste water;

  • aqueous extracts and leachates;

  • eluates of sediments (fresh water);

  • pore water;

  • aqueous solutions of single substances or of chemical mixtures;

  • drinking water.

The limit of quantification (LOQ) of this method for the direct analysis of water samples is between 8 ng/l and 15 ng/l 17β-estradiol equivalents (EEQ) based on the results of the international interlaboratory trial (see Annex F). The upper threshold of the dynamic range for this test is between 120 ng/l and 160 ng/l 17β-estradiol equivalents (EEQ). Samples showing estrogenic potencies above this threshold have to be diluted for a valid quantification. Extraction and pre-concentration of water samples can prove necessary, if their estrogenic potential is below the given LOQ.