BS ISO 19040-1:2018
Water quality. Determination of the estrogenic potential of water and waste water Yeast estrogen screen (<i>Saccharomyces</i> <i>cerevisiae</i>)
Standard number: | BS ISO 19040-1:2018 |
Pages: | 60 |
Released: | 2018-08-10 |
ISBN: | 978 0 580 84720 2 |
Status: | Standard |
BS ISO 19040-1:2018
This standard BS ISO 19040-1:2018 Water quality. Determination of the estrogenic potential of water and waste water is classified in these ICS categories:
- 13.060.70 Examination of biological properties of water
This document specifies a method for the determination of the estrogenic potential of water and waste water by means of a reporter gene assay with genetically modified yeast strains Saccharomyces cerevisiae. This reporter gene assay is based on the activation of the human estrogen receptor alpha.
This method is applicable to:
fresh water;
waste water;
aqueous extracts and leachates;
eluates of sediments (fresh water);
pore water;
aqueous solutions of single substances or of chemical mixtures;
drinking water.
The limit of quantification (LOQ) of this method for the direct analysis of water samples is between 8 ng/l and 15 ng/l 17β-estradiol equivalents (EEQ) based on the results of the international interlaboratory trial (see Annex F). The upper threshold of the dynamic range for this test is between 120 ng/l and 160 ng/l 17β-estradiol equivalents (EEQ). Samples showing estrogenic potencies above this threshold have to be diluted for a valid quantification. Extraction and pre-concentration of water samples can prove necessary, if their estrogenic potential is below the given LOQ.