Ecotoxicity tests can be applied to wastes to identify their potential hazardous properties with respect to the environment or to assess the risk related to a site-specific exposure scenario. This document provides guidance for the selection and use of ecotoxicity tests for both applications. This document focuses on the following selected field of applications: a) hazardous properties (i.e. HP 14); b) Site-specific exposure scenario; c) Landfill management: a. monitoring of leachates; b. mineral waste going to non-controlled landfill sites. d) Re-use of waste: c. use of sludge in agriculture; d. use of mineral waste in road construction. Other fields of application can also be covered by ecotoxicological testing not being in the scope of the document. The ecotoxicological assessment of waste within other scenarios might need the development of other test strategies. With regard, more specifically, to the assessment of hazardous properties, this document focuses on the ecotoxicological characterization of waste using biotests. Depending on the waste type and the assessment goal, relevant criteria are described for the selection of a test strategy and the suitable ecotoxicity test(s). This document also provides guidance for ecotoxicity test protocols to meet the specific demands of waste testing (e.g., limitations, test design, confounding factors). The proposed tests represent a minimum test battery that can be completed by additional tests or even be replaced by others according to the waste, the intended use or protection goal envisaged.