This document specifies performance criteria for the selection of single-laboratory validated or collaborative study validated methods of analysis of mycotoxins in feed. The terms and definition of the relevant parameters for method validation are included. The performance requirements and characteristics are provided. This document could serve as a guide: to assess the quality of new European Standard methods under validation; to review the quality of previous collaborative trials; to confirm the extension of the scope of an already published European Standard applied to other analyte concentrations or matrices; or to evaluate the fitness-for-purpose of single-validated methods. The performance criteria can apply to methods dedicated to the determination of mycotoxins.