UNE EN 17270:2020
Animal feeding stuffs: Methods of sampling and analysis - Determination of theobromine in feed materials and compound feed, including cocoa derived ingredients, by liquid chromatography
Alimentos para animales. Métodos de muestreo y análisis. Determinación de la teobromina en piensos y piensos compuestos, incluidos los ingredientes derivados del cacao, mediante cromatografía líquida.
| Standard number: | UNE EN 17270:2020 |
| Pages: | 26 |
| Released: | 2020-07-22 |
| Status: | Standard |
| Pages (Spanish): | 27 |
UNE EN 17270:2020
This document method is applicable for the determination of theobromine in compound feed by liquid chromatography with UV detection in the tested range of 27 mg/kg to 307 mg/kg. This method has been validated using complementary compound feed for adult dogs and complementary compound feedstuff for horses. The actual working range can extend beyond the tested range. Alternative chromatography conditions using liquid chromatography tandem mass spectrometry (LC-MS/MS) are also provided for the validated range of 49 mg/kg to 307 mg/kg. This method has also been shown to be fit for purpose for the determination of theobromine in baking chocolate by both HPLC-UV and LC-MS/MS.