This document specifies a screening method for the detection of staphylococcal enterotoxins SEA, SEB, SECs, SED and SEE (SEA to SEE) in the food stuff. The Standard consists of two main steps (i) extraction followed by a concentration based on dialysis principle and (ii) an immuno-enzymatic detection using commercially available detection kits. This International Standard is applicable for the screening of staphylococcal enterotoxins (SEA-SEE) in products intended for human consumption. Other staphylococcal enterotoxins such as types SEG, SEH, SEI, SER, SES and SET can also cause illness. However due to the lack of commercially available detection kit this Standard only focuses on types SEA to SEE.