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Homepage>ASTM Standards>ASTM D5296-19 - Standard Test Method for Molecular Weight Averages and Molecular Weight Distribution of Polystyrene by High Performance Size-Exclusion Chromatography
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Released: 2020-01-06

ASTM D5296-19

Standard Test Method for Molecular Weight Averages and Molecular Weight Distribution of Polystyrene by High Performance Size-Exclusion Chromatography

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Language:English
Released:2020-01-06
Pages:13
Standards number:ASTM D5296-19
Standard number:D5296-19
Released:01.11.2019
Status:Active
Pages:13
Section:08.03
Keywords:high-performance size exclusion chromatography (HPSEC); molecular weight average; molecular weight distribution; polystyrene;
DESCRIPTION

1.1 This test method covers the determination of molecular weight (MW) averages and the distribution of molecular weights for linear, soluble polystyrene by high-performance size-exclusion chromatography (HPSEC). This test method is not absolute and requires the use of commercially available narrow molecular weight distribution (MWD) polystyrene standards for calibration. This test method is applicable for samples containing molecular weight components that have elution volumes falling within the elution volume range defined by polystyrene standards (that is, molecular weights generally from 2000 to 2 000 000 g·mol−1).

1.2 The HPSEC is differentiated from traditional size-exclusion chromatography SEC (also referred to as gel permeation chromatography (GPC)) in that the number of theoretical plates per metre with an HPSEC system is at least ten times greater than that for traditional SEC (see Terminology D883 and Practice D3016).2 The HPSEC systems employ low-volume liquid chromatography components and columns packed with relatively small (generally 3 to 20 μm) microporous particles. High-performance liquid chromatography instrumentation and automated data handling systems for data acquisition and processing are required.

1.3 The values stated in SI units are to be regarded as the standard.

1.4 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety, health, and environmental practices and determine the applicability of regulatory limitations prior to use. Specific precautionary statements are given in Section 9.

Note 1: There is no known ISO equivalent to this standard.

1.5 This international standard was developed in accordance with internationally recognized principles on standardization established in the Decision on Principles for the Development of International Standards, Guides and Recommendations issued by the World Trade Organization Technical Barriers to Trade (TBT) Committee.