BS EN 17683:2023

Animal feeding stuffs. Methods of sampling and analysis. Determination of pyrrolizidine alkaloids in animal feeding stuff by LC-MS/MS

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Standard number:	BS EN 17683:2023
Pages:	70
Released:	2023-03-09
ISBN:	978 0 539 05201 5
Status:	Standard

BS EN 17683:2023 Animal feeding stuffs. Methods of sampling and analysis. Determination of pyrrolizidine alkaloids in animal feeding stuff by LC-MS/MS

Introducing the BS EN 17683:2023, a comprehensive guide to the methods of sampling and analysis of animal feeding stuffs, with a specific focus on the determination of pyrrolizidine alkaloids using LC-MS/MS. This standard is an essential tool for anyone involved in the production, testing, and regulation of animal feed, ensuring the highest levels of safety and quality.

Released on 2023-03-09, this standard is the latest in a series of guidelines designed to ensure the safety and quality of animal feed. With 70 pages of detailed instructions and guidelines, it provides a comprehensive overview of the methods used to sample and analyze animal feed, with a specific focus on the detection of pyrrolizidine alkaloids.

Why Choose BS EN 17683:2023?

Pyrrolizidine alkaloids are naturally occurring toxins that can be found in a variety of plants. When ingested by animals, they can cause serious health problems, including liver damage and even death. Therefore, it is crucial to detect and quantify these toxins in animal feed to ensure the safety of livestock.

The BS EN 17683:2023 provides a reliable and accurate method for the detection of these toxins using LC-MS/MS, a powerful analytical technique that combines the physical separation capabilities of liquid chromatography (LC) with the mass analysis capabilities of mass spectrometry (MS). This method allows for the precise identification and quantification of pyrrolizidine alkaloids, ensuring the safety and quality of animal feed.

Key Features of BS EN 17683:2023

The BS EN 17683:2023 is packed with valuable information and guidelines, including:

A detailed overview of the sampling methods used in the analysis of animal feed.
Comprehensive instructions on how to perform the LC-MS/MS analysis of pyrrolizidine alkaloids.
Guidelines on how to interpret the results of the analysis and determine the safety of the feed.

With its clear instructions and detailed guidelines, the BS EN 17683:2023 is an invaluable resource for anyone involved in the production, testing, and regulation of animal feed.

Get Your Copy Today

Don't compromise on the safety and quality of your animal feed. Get your copy of the BS EN 17683:2023 today and ensure that your feed is free from harmful pyrrolizidine alkaloids. With its ISBN 978 0 539 05201 5, you can easily find and order this standard from any major book retailer or directly from the British Standards Institution.

Remember, the safety of our livestock depends on the quality of their feed. Make sure you're doing everything you can to ensure their health and wellbeing with the BS EN 17683:2023.

DESCRIPTION

BS EN 17683:2023

This standard BS EN 17683:2023 Animal feeding stuffs. Methods of sampling and analysis. Determination of pyrrolizidine alkaloids in animal feeding stuff by LC-MS/MS is classified in these ICS categories:

65.120 Animal feeding stuffs

This document describes a method for the quantitative determination of pyrrolizidine alkaloids (PA) in complete and supplementary feed and in forages by liquid chromatography tandem mass spectrometry (LC-MS/MS) after solid phase extraction (SPE) clean-up. The method has been successfully validated in a collaborative trial for the matrices complete feed for horses, supplementary feed for horses, supplementary feed for rodents, hay, alfalfa and grass silage. Validation was carried out for the PA and concentrations ranges listed in Table 1. It was demonstrated that the PA isomeric pairs senecivernine and senecionine as well as senecivernine-N-oxide and senecionine-N-oxide cannot be determined individually due to insufficient chromatographic separation. However, the sums of the individual PA of the isomeric pairs were quantified with sufficient reproducibility. Co-elution of other PA-isomers not included in the scope of the method shall be taken into account. A list of potentially co-eluting isomers is presented in Annex E. Although the calibration range of the method protocol is specified from 10 μg/kg to 300 μg/kg, the results of the collaborative study showed, that the dilution of sample extracts with blank sample extracts enables for the quantitation of concentrations exceeding the calibration range. Satisfactory reproducibility was achieved when quantifying up to 1428 μg/kg for individual PA and up to 887 μg/kg for the sum of isomeric pairs. NOTE 1 A second method was part of the method validation collaborative main trial. For this method PA-N-Oxides are reduced by adding zinc powder to the extract of the feed material. The following steps correspond to the first and main method. Quantitative results for each PA except the otonecine type PA senkirkine represent the sum of the free PA base and its corresponding N-oxide. NOTE 2 Due to insufficient numbers of data for some analyte-matrix combinations statistical evaluation was not valid for standardization. Received data indicated the methods applicability in experienced laboratories with appropriate quality assurance measures. Therefore, the method description is included as an informative annex (Annex D).

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