This document specifies a test method by acetonitrile solvent extraction for the determination of the total content (solvent extractible) of the following preservative agents in leather by liquid chromatography:

— 2-(thiocyanomethylthio)-benzothiazole (TCMTB);

— 4-chloro-3-methylphenol (PCMC);

— 2-phenylphenol (OPP);

— 2-octylisothiazol-3(2H)-one (OIT);

This method can also be used to determine breakdown products of these preservative agents, which protect leather from microbiological attack.