UNE EN ISO 18363-4:2022
Animal and vegetable fats and oils - Determination of fatty-acid-bound chloropropanediols (MCPDs) and glycidol by GC/MS - Part 4: Method using fast alkaline transesterification and measurement for 2-MCPD, 3-MCPD and glycidol by GC-MS/MS (ISO 18363-4:2021)
Aceites y grasas de origen animal y vegetal. Determinación de ésteres de cloropropanodioles (MCPD) y sus ácidos grasos y ésteres glicidílicos y sus ácidos grasos mediante GC/MS. Parte 4: Método basado en la transesterificación alcalina rápida y medición de 2-MCPD, 3-MCPD y glicidol mediante GC-MS/MS. (ISO 18363-4:2021).
| Standard number: | UNE EN ISO 18363-4:2022 |
| Pages: | 33 |
| Released: | 2022-05-18 |
| Status: | Standard |
| Pages (Spanish): | 36 |
UNE EN ISO 18363-4:2022
This part of ISO 18363 describes a rapid procedure for the simultaneous determination of 2-MCPD esters (bound 2-MCPD), 3 MCPD esters (bound 3 MCPD) and glycidyl esters (bound glycidol) in a single assay, based on alkaline catalysed ester cleavage and derivatization of cleaved (free) analytes with phenylboronic acid (PBA) prior to GC-MS/MS analysis. This method is applicable to solid and liquid fats and oils. This part of ISO 18363 can also apply to animal fats and used frying oils and fats, but a validation study must be undertaken before the analysis of these matrices. For all three analytes the limit of quantification (LOQ) is 0,1 mg/kg and the limit of detection (LOD) is 0,03 mg/kg. Milk and milk products (or fat coming from milk and milk products), infant formulas, emulsifiers, free fatty acids and other fats and oils derived matrices are excluded from the scope of this international standard.