BS EN 14526:2025

This standard BS EN 14526:2025 Foodstuffs. Determination of saxitoxin-group toxins in shellfish. HPLC method using pre-column derivatization with peroxide or periodate oxidation is classified in these ICS categories:

• 67.120.30 Fish and fishery products

This document specifies a method [1] for the quantitative determination of saxitoxin (STX), decarbamoyl saxitoxin (dcSTX), neosaxitoxin (NEO), decarbamoyl neosaxitoxin (dcNEO), gonyautoxin 1 and 4 (GTX1,4; sum of isomers), gonyautoxin 2 and 3 (GTX2,3; sum of isomers), gonyautoxin 5 (GTX5), gonyautoxin 6 (GTX6), decarbamoyl gonyautoxin 2 and 3 (dcGTX2,3; sum of isomers), N-sulfocarbamoyl gonyautoxin 2 and 3 (C1,2; sum of isomers) and N-sulfocarbamoyl gonyautoxin 1 and 4 (C3,4; sum of isomers) in (raw) mussels, oysters, scallops and clams. Laboratory experience has shown that this document can also be applied to other marine invertebrates [2], [3] and processed products of those species, however, no complete interlaboratory validation study according to ISO 5725-2 [21] has been carried out so far. The method described was validated in an interlaboratory study [4], [5] and was also verified in a European Union Reference Laboratory for Marine Biotoxins (EURLMB)-performance test aiming the total toxicity of the samples [6]. Toxins which were not available in the first interlaboratory study [4], [5] as dcGTX2,3 and dcNEO were validated in two additional interlaboratory studies [7], [8]. The lowest validated levels [4], [5], [8], are given as mass fraction of toxin (free base) in µg/kg shellfish tissue and also as µmol/kg shellfish tissue and are listed in Table 1. [Table 1 - Lowest validated levels] A quantitative determination of GTX6 was not included in the first interlaboratory study but several laboratories detected this toxin directly after solid phase extraction with ion-exchange (SPE-COOH) clean-up and reported a mass fraction (free base) of 30 µg/kg or higher in certain samples. For that reason, the present method is applicable to quantify GTX6 directly, depending on the availability of the standard substance. Whenever GTX6 standard is not commercially available, it is possible to determine GTX6 after hydrolysis of Fraction 2 of the SPE-COOH clean-up, described in 7.4, as NEO. The indirect quantification of GTX6 was validated in two additional interlaboratory studies [7], [8]. A study to compare direct and indirect GTX6 quantification was conducted at the EURLMB [16]. A quantitative determination of C3,4 was included in the first interlaboratory study. The present method is applicable to quantify C3,4 directly, depending on the availability of the standard substance. If no standard substances are available, C3,4 can only be quantified as GTX1,4 if the same hydrolysis protocol used for GTX6 (7.4) is applied to Fraction 1 of the SPE-COOH clean-up [10]. A study to compare direct and indirect C3,4 quantification was conducted at the EURLMB [16].

BS EN 14526:2025 - Foodstuffs: Determination of Saxitoxin-Group Toxins in Shellfish

Introducing the BS EN 14526:2025, a comprehensive standard that provides a detailed methodology for the determination of saxitoxin-group toxins in shellfish. This standard is essential for laboratories and food safety professionals who are committed to ensuring the safety and quality of shellfish products.

Overview

The BS EN 14526:2025 standard outlines a precise and reliable method for detecting saxitoxin-group toxins in shellfish using High-Performance Liquid Chromatography (HPLC). This method employs pre-column derivatization with either peroxide or periodate oxidation, ensuring accurate and reproducible results.

Key Features

• Standard Number: BS EN 14526:2025
• Pages: 80
• Release Date: April 14, 2026
• ISBN: 978 0 539 32875 2
• Status: Standard

Why Choose BS EN 14526:2025?

This standard is indispensable for those involved in the food industry, particularly in the testing and analysis of shellfish. Saxitoxins are potent neurotoxins that can pose significant health risks if consumed. Therefore, accurate detection and quantification are crucial for consumer safety and regulatory compliance.

Benefits of Using This Standard:

• Enhanced Safety: By following the guidelines set out in this standard, laboratories can ensure that shellfish products are free from harmful levels of saxitoxins, protecting consumers from potential health hazards.
• Regulatory Compliance: Adhering to this standard helps businesses comply with national and international food safety regulations, avoiding potential legal issues and enhancing their reputation.
• Scientific Rigor: The HPLC method with pre-column derivatization is a scientifically validated approach, providing confidence in the accuracy and reliability of test results.

Detailed Methodology

The BS EN 14526:2025 standard provides a step-by-step guide to the HPLC method, including sample preparation, derivatization procedures, and analytical conditions. The use of peroxide or periodate oxidation in the pre-column derivatization process is a key feature, enhancing the detection sensitivity and specificity for saxitoxin-group toxins.

Sample Preparation:

Proper sample preparation is critical for accurate toxin detection. The standard outlines the necessary steps to ensure that samples are prepared consistently and effectively, minimizing the risk of contamination or error.

Derivatization Process:

The pre-column derivatization process involves the oxidation of saxitoxins using either peroxide or periodate. This step is crucial for converting the toxins into detectable forms, allowing for precise quantification through HPLC analysis.

Analytical Conditions:

The standard specifies the optimal conditions for HPLC analysis, including column type, mobile phase composition, flow rate, and detection wavelength. These parameters are designed to maximize the separation and detection of saxitoxin-group toxins.

Who Should Use This Standard?

The BS EN 14526:2025 standard is ideal for:

• Food Safety Laboratories: Ensuring the safety of shellfish products through rigorous testing.
• Regulatory Bodies: Establishing and enforcing food safety standards and regulations.
• Shellfish Producers: Maintaining product quality and safety to meet consumer and regulatory expectations.

Conclusion

The BS EN 14526:2025 standard is a vital resource for anyone involved in the testing and analysis of shellfish. By providing a detailed and scientifically validated method for detecting saxitoxin-group toxins, this standard helps ensure the safety and quality of shellfish products, protecting consumers and supporting industry compliance with food safety regulations.

Invest in the BS EN 14526:2025 standard today to enhance your laboratory's testing capabilities and ensure the highest standards of food safety.